Products
The Lucid™ Profiling Access Pack
The Lucid Profiling Access Pack provides a proven path to top-down biomarker discovery.
- High-throughput, high-resolution native protein and peptide profiling across a broad mass range (typically 1.5–70 kD)
- Chromatographic ProteinChip arrays amenable to diverse biological samples or sample fractions
- Multiple modes of separation expand proteomic coverage
- Easy-to-use tools within the Lucid proteomics software for sample tracking and univariate and multivariate data mining
- Consulting services with a team of Lucid proteomics specialists, providing recommendations on study design, sample selection, experimental protocols, and statistical analyses for one biomarker discovery study
The Lucid profiling access pack (catalog #LPS-0020) includes: Lucid array holders and stand, Lucid proteomics users license, profiling consulting agreement, Lucid system qualification kit, starter kit, Lucid proteomics software, profiling handbook, profiling roadmap, ProteinChip CM10 arrays and low-stringency buffer, ProteinChip H50 arrays and buffer, ProteinChip IMAC30 arrays and buffers, Protein Chip Q10 arrays and buffer, ProteinChip SPA matrix , ProteinChip CHCA matrix , ProteinChip cassette-compatible bioprocessors, and a handheld bar code reader.
Also available, the Lucid profiling basics pack (catalog #LPS-0021) includes an array holder and stand, a profiling users license, and the system qualification kit.
Lucid™ ID Access Pack
The Lucid ID Access Pack provides a clear path to identification of native protein and peptide biomarkers.
- Tools for native protein purification, enrichment, and digestion after top-down proteomic profiling
- ProteinChip arrays and consumables to screen purification conditions and monitor purification processes prior to SDS-PAGE, digestion, and identification by MS/MS
- On-chip enrichment and potential direct identification by MS/MS of peptide targets below 4 kD
- Consulting services with a team of Lucid proteomics specialists, providing recommendations and suggested protocols on purification and identification of up to five protein or peptide targets within a single project
The Lucid ID access pack (catalog #LPS-0010) includes: Lucid array holder and stand, Lucid proteomics user license, ID consulting agreement, ProteinChip acetonitrile and TFA (2 ml each), ProteinChip NP20 arrays (includes 12 ProteinChip arrays), ProteinChip assortment pack (includes 3 each of H50, CM10, IMAC30 and Q10 ProteinChip arrays), ProteinChip cassette-compatible bioprocessor, ProteinChip CM10 low-stringency buffer (200 ml), ProteinChip IMAC buffer set (includes 30 ml IMAC charging solution, 30 ml neutralizing solution, 200 ml IMAC binding buffer), and a ProteinChip matrix kit.
Lucid™ System Qualification Kit
The Lucid system qualification kit provides prepared methods for evaluation, optimization, and monitoring of instrument performance.
- The Bruker flexControl profiling methods calibrated to premade ProteinChip arrays, methods, and scripts, in order to achieve optimum results for protein detection
- Tests for detector gain, laser stability, laser energy optimization, mass accuracy, and sensitivity
- Standardized and optimized system and data collection methods
- Minimal instrument-based analytical bias, promoting the reproducibility of results over time
- Critical first step for biomarker discovery studies
- Also offered with Lucid profiling packages
The Lucid system qualification kit (catalog #LPS-030) includes: 1 detector calibration array, 1 peptide standard array, 3 premade CM10 arrays, and an instruction manual. The included consumables are sufficient for six months of system qualification testing.
The system qualification kit (cat# LPS-030) includes: 1 detector calibration array, 1 peptide standard array, 3 premade CM10 arrays, and an instruction manual. The included consumables are sufficient for six months of system qualification testing.
Lucid™ Proteomics Software
Lucid proteomics software provides sample tracking, data collection, and data analysis within a single package.
- Higher throughput data collection runs by assigning Bruker’s autoXecute methods to ProteinChip arrays
- Virtual notebooks to track sample properties and experimental parameters for critical data organization and annotation
- Intuitive biomarker-driven data analysis tools, including multiple modes of spectral visualization, easily generated P value and receiver operator characteristic (ROC) statistics, trend plots, hierarchical clustering, and principle component analysis (PCA)
- Built-in analyses of system qualification tests track and ensure instrument reliability over time.
The Lucid Proteomics Software (cat# LPS-0050) includes two USB keys.
Bio-Rad ProteinChip Arrays
For sample partitioning, concentration, and cleanup.
- The ProteinChip arrays play a key role in the Lucid Proteomics System.
- The chromatographic nature of the surface-enhanced laser desorption/ionization (SELDI) surface allows direct analysis of complex biological samples across a broad mass range.
- It does so in a robust, reproducible manner to quickly deliver protein profiles for biomarker discovery.
Bruker Mass Spectrometers
For high-performance sample analysis.
- A system approach as advanced as the Lucid Proteomics System deserves an advanced mass spectrometer.
- The ultrafleXtreme™ is capable of 1 kHz speed in both TOF and TOF/TOF modes, providing high performance and extreme flexibility.
- The Lucid Proteomics System is also compatible with autoflex™ and ultraflex™ TOF and TOF/TOF models.
